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ASTM D4012-15
水中微生物三磷酸腺苷 (ATP) 含量的標準試驗方法

Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water


標準號
ASTM D4012-15
發布
2015年
總頁數
9頁
發布單位
美國材料與試驗協會
替代標準
ASTM D4012-23
當前最新
ASTM D4012-23a
 
 
引用標準
ASTM D1129 ASTM D1193 ASTM D1601 ASTM D4175 ASTM D5847 ASTM D6161 ASTM D6300 ASTM D7687 ASTM E2694 ASTM F1671
適用范圍

5.1 A rapid and routine procedure for determining biomass of the living microorganisms in cultures, waters, wastewaters, and in plankton and periphyton samples taken from surface waters is frequently of vital importance. However, classical techniques such as direct microscope counts, turbidity, organic chemical analyses, cell tagging, and plate counts are expensive, time-consuming, or tend to underestimate total numbers. In addition, some of these methods do not distinguish between living and nonliving cells.

5.2 This test method measures the concentration of cellular-ATP present in the sample. ATP is a constituent of all living cells, including bacteria, algae, protozoa, and fungi. Consequently, the presence of cellular-ATP is an indicator of total metabolically active microbial contamination in water. ATP is not associated with matter of non-biological origin.

5.3 The ATP (luciferin-luciferase) method is a rapid, sensitive determination of viable microbial biomass. ATP is the primary energy donor for life processes, does not exist in association with nonliving detrital material, and the amount of ATP per unit of biomass (expressed in weight) is relatively constant. (ATP per cell varies with species and physiological state of the organism.)

5.4 This test method can be used to:

5.4.1 Estimate viable microbial biomass in cultures, waters, and wastewaters.

5.4.2 Estimate the amount of total viable biomass in plankton and periphyton samples.

5.4.3 Estimate the number of viable cells in a unispecies culture if the cATP content (or if the average amount of cATP) per cell is known.

5.4.4 Estimate and differentiate between zooplanktonic, phytoplanktonic, bacterial, and fungal cATP through size fractionation of water, and wastewater samples.

5.4.5 Measure the mortality rate of microorganisms in toxicity tests in entrainment studies, and in other situations where populations or assemblages of microorganisms are placed under stress.

5.5 This test method is similar to Test Methods D7687 and E2694 except for the volumes sampled, and omission of wash and drying steps used in Test Methods D7687 and E2694 to remove interferences (1.3).

5.6 Although ATP data generally covary with culture data in water samples, different factors affect cATP concentration than those that affect culturability.

5.6.1 Culturability is affected primarily by the ability of captured microbes to proliferate on the growth medium provided, under specific growth conditions. Consequently, a proportion of the active or inactive microbial population present in a sample......


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磷酸 測試方法


BE-NBN

NBN 682-1965 磷酸鹽測定 NBN V 21-007-1977 奶油中磷酸酶活性的測定 NBN T 03-268-1980 工業用磷酸(包括食品).鈣含量的測定.火焰原子吸收法 NBN T 03-267-1980 工業用磷酸(包括食品),所有磷的氧化物的測定.喹啉.鉬酸鹽重力測定法 NBN T 03-261-1980 工業用磷酸,鈣含量的測定.容積法 NBN T 03-295-1980 工業用三聚磷酸鈉和焦磷酸鈉,機械篩分后顆粒分布的測定 NBN T 03-298-1980 工業用三聚磷酸鈉,三聚磷酸鹽的估算.三乙二胺 氯化鈷重力測定法 NBN T 03-297-1980 工業用焦磷酸鈉,焦磷酸鹽含量的測定.電勢法 NBN T 03-292-1980 工業用三聚磷酸鈉,pH值測量.電勢法 NBN T 03-269-1980 工業用磷酸(包括食品).氯含量的測定.電勢法 NBN T 03-264-1980 工業用磷酸,砷含量的測定.二乙基二硫代氨基甲酸銀.光度測定法 NBN T 03-262-1980 工業用磷酸,鐵含量的測定.2,2'聯吡啶分光光度法 NBN T 03-296-1980 工業用三聚磷酸鈉和焦磷酸鈉,磷酸鹽含量的測定.使用還原的磷鉬酸鹽進行光度測定法 NBN T 03-299-1980 工業用三聚磷酸鈉和焦磷酸鈉,所有磷的氧化物含量的測定.喹啉,磷鉬酸鹽重力測定法 NBN T 03-313-1980 工業用縮聚磷酸鹽(包括食品).氮的氧化物含量的測定.3,4二甲苯酚分光光度測定法 NBN T 03-265-1980 工業用磷酸磷酸鈉(包括食品),氟含量的測定.茜素氨絡和硝酸鑭光度測定法 NBN T 03-266-1980 工業用磷酸,可溶性硅石含量的測定.被還原的分光光度分析法 NBN T 03-300-1980 工業用三聚磷酸鈉和焦磷酸鈉,柱狀色譜分離法,不同磷酸鹽形式的測定 NBN T 03-263-1980 工業用磷酸,硫酸鹽含量的測定.還原和滴定分析法 NBN T 03-270-1980 工業用磷酸(包括食品),氮的氧化物含量的測定.3,4-二甲苯酚分光光度測定法 NBN T 03-294-1980 工業用三聚磷酸鈉和焦磷酸鈉.燒失量的測定 NBN T 03-293-1980 工業用三聚磷酸鈉和焦磷酸鈉,鐵含量的測定.2,2'-聯吡啶分光光度測定法 NBN T 03-291-1980 工業用三聚磷酸鈉.不溶于水物質的測定 NBN T 03-311-1980 工業用縮聚磷酸鹽(包括食品),砷含量的測定.二乙基二硫代氨基甲酸銀,光度測定法 NBN T 03-312-1980 工業用縮聚磷酸鹽(包括食品),氯含量的測定.電勢法 NBN T 04-247-1983 工業用磷酸三甲苯酯,紅色苯酚酸度的測定.容積法 NBN T 03-526-1983 工業用六氟硅酸鈉.磷酸鹽含量的測定.游離酸度和所有六氟硅酸含量的測定.滴定法 NBN T 03-527-1983 工業用六氟硅酸鈉.磷酸鹽含量的測定: NBN T 04-248-1983 工業用磷酸三甲苯酯,表面游離酚含量的測定.容積法 NBN T 04-246-1983 工業用磷酸三甲苯酯.試驗方法列表 NBN V 21-032-1983 牛奶 磷酸酶活性的測定 NBN T 03-314-1984 工業用縮聚磷酸鹽(包括食品),鈣含量的測定.火焰原子吸收法 NBN T 03-274-1985 工業用磷酸(包括食品),硫化氫含量的測定.滴定法 NBN T 03-275-1985 工業用磷酸(包括食品).釩含量的測定 NBN T 03-273-1985 工業用磷酸,較高可溶硫酸鹽含量的測定.還原后滴定法




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